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Chromosomal distribution of the As51 satellite DNA in two species complexes of the genus Astyanax (Pisces, Characidae) Genet. Mol. Biol.
Abel,Luciano Douglas dos Santos; Mantovani,Monique; Moreira-Filho,Orlando.
The chromosomal localization of the As51 satellite DNA was identified by fluorescent in situ hybridization (FISH) in specimens of the characid fish Astyanax scabripinnis and Astyanax fasciatus, which are considered species complexes because of their extensive karyotypical and morphological variability. A conserved chromosomal distribution of the As51 satellite, coincident with distal C-banded segments was demonstrated. The alternative interstitial localization of this satellite DNA and possible alterations of its structure suggest that this sequence underwent quantitative, positional and structural variations, as the A. scabripinnis and A. fasciatus complexes diverged.
Tipo: Info:eu-repo/semantics/article Palavras-chave: As51; Astyanax scabripinnis; Astyanax fasciatus; Fluorescence in situ hybridization (FISH); C-banding; Satellite DNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300008
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Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae) Genet. Mol. Biol.
Mantovani,Monique; Abel,Luciano Douglas dos Santos; Mestriner,Carlos Alberto; Moreira-Filho,Orlando.
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Satellite DNA; Chromosome structure; Base-specific fluorochromes; Heterochromatin heterogeneity; Fluorescence in situ hybridization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012
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